Development of a One-Step Real-Time TaqMan Reverse Transcription Polymerase Chain Reaction (RT-PCR) Assay for the Detection of the Novel Variant Infectious Bursal Disease Virus (nVarIBDV) Circulating in China
نویسندگان
چکیده
The novel variant IBDV (nVarIBDV, especially genotype A2dB1) mainly affects broilers in China. It causes an infection characterized by the atrophy of bursa, a decrease level lymphocytes, proliferation fibrous tissue around follicle, and severe follicle bursa. Poultry vaccinated with live vaccines do not have challenge present bursa atrophy, which is misdiagnosed for nVarIBDV because lack other gross clinical symptoms. study sought to explore potential reliability real-time TaqMan analysis method detection discrimination from that non-nVarIBDV, vaccine strains. This will help monitor poultry control manage IBDVs. nucleotide polymorphism 5′-UTR region vp5/vp2 overlapping segment A sequences were used establish one-step reverse transcription polymerase chain reaction (RT-PCR) this study. results showed accurately distinguished non-nVarIBDV strains (especially strains), there no cross-reactions infectious bronchitis virus (IBV), Newcastle disease (NDV), avian influenza (AIV), laryngotracheitis (ILTV), fowlpox (FPV), Mycoplasma gallisepticum (M. gallisepticum), synoviae synoviae), IBDV-negative field samples. linear dynamic range between 102 107 DNA copies/reaction, average R2 0.99 efficiency 93% 1.00 94% non-nVarIBDV. was also 84 bursae chickens vaccine. (vaccine compared strategy based on sequence HVRs at vp2 gene or PCR vp5 gene. These findings RT-PCR provides rapid, sensitive, specific, simple approach infections caused useful diagnostic tool identifying distinguishing
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ژورنال
عنوان ژورنال: Viruses
سال: 2023
ISSN: ['1999-4915']
DOI: https://doi.org/10.3390/v15071453